VitriBlast Overview

VitriBlast™ and ThermoBlast™ vitrification media optimised for blastocysts are based on well tested standard formulations (Lane et al). Since the formulations have been used considerably, Nidacon can provide you with a very detailed protocol for vitrifying blastocysts, just for your security.

Why Vitrification?

The main problem when freezing cells is the formation of intracellular ice crystals during both cooling and warming, since these ice crystals have a detrimental effect on cell survival. Vitrification, the extremely rapid freezing of cellular material, makes it possible to freeze cells without forming ice crystals within the cells. The result of vitrification is a very homogenous structure, an amorphous crystalline structure, as seen with glass and obsidian, materials which fracture easily, have sharp edges and smooth surfaces (glass transition). The cryoprotectants used for vitrification are a combination of Dimethyl Sulfoxide (DMSO), Ethylene Glycol, Sucrose and Ficoll. Nidacon’s VitriBlast™ for cooling and ThermoBlast™ for warming, can be used with different types of vitrification-devices (cryo-loop, cryo-top and others).

Why Nidacon Vitrification?

VitriBlast™ and ThermoBlast™ are based on clinically, well tested, standard formulations (Lane et al). Numerous publications demonstrate their effectiveness regarding both survival of blastocysts and pregnancy rates. In addition, follow-up studies have been published on live-births, where a comparison is made with babies from fresh blastocysts, slow-frozen early cleavage stage embryos and vitrified blastocysts (Wikland M et al.(2010)).

Which vitrification device should I use?

Since there are so many different vitrification devices on the market today, Nidacon decided not to manufacture its own device but to make sure that its own product, VitriBlast™ Kit, can be used together with all different devices.

VitriBlast™Kit and ThermoBlast™Kit were developed together with Fertility Centre in Gothenburg,Sweden and they have been using the cryoloop very successfully for quite some time. In our workshops and instructions for use, the cryoloop is therefore often used or referred to, although but VitriBlast™Kit can be used with other devices following the same instructions.

In some countries you are not allowed to use an open system and, therefore, the high security straw can be an option. We have listed below some of the more common devices and information on where to buy them.

  1. CR Kitazato Cryotop (pack of 10),
  2. HSV High Security Vitrification kit,
  3. Cryoloop (Vitroloop),
  4. CryoLock,

Clinical results

The Fertility Centre at Carlanderska Hospital in Gothenburg, Sweden, has been using this formulation since 2005 with excellent results. Blastocyst survival rates are 5% higher (84% vs 80%), and pregnancy rate per transfer is much higher (54% vs 37%), when compared to the slow-freezing technique for cleavage stage embryos.

Quality control testing

Each batch is tested for endotoxin levels, sterility, mouse embryo assay, pH and osmolality.


  • Sodium chloride
  • Potassium chloride
  • Magnesium sulphate
  • Potassium dihydrogen phosphate
  • Sodium bicarbonate
  • hSA Human serum albumin
  • Purified water
  • Glucose
  • Calcium lactate
  • Sodium pyruvate
  • EDTA
  • Sucrose
  • Ethylene glycol
  • DMSO
  • Ficoll

Performance Characteristics

pH: 7.25-7.45
Endotoxin levels: <0.5 EU/mL
MEA: Reexpanded blastocysts after exposure >80%

Bottles and screw caps are M.E.A. tested

Storage and Stability

Store at 2 to 30°C and avoid temperatures above or below these values. Under these conditions VitriBlast™ has a shelflife of 12 months. The expiry date is shown on both bottles and cartons.

Open and close bottles under aseptic conditions. After opening store at 2 to 8°C when not in use. Shelf-life on the product label applies when the product is stored according to manufacturer’s recommendations. No antibiotics, unstable additives or preservatives have been added by the manufacturer to VitriBlast™.

Precautions and Warnings

  • Use aseptic procedures at all times
  • Do not use any vial or solution that shows evidence of particulate matter or cloudiness
  • VitriBlast™ contains DMSO which is highly pentetrable substance. A material safety data sheet is available from the distributor or manufacturer (see
  • VitriBlast™ contains Ethylene glycol which is toxic when ingested.
  • Closed Vitrification system is mandatory in USA
  • Federal Law (USA) restricts this device to sale by or on the order of a physician
  • Please check for regulatory compliance governing the use of ART products in your country

Ordering Information

Article No. / Name / Volume
VBK-010 / VitriBlast™ Kit / 3x10 mL

copyright © 2001 - 2019 all rights reserved